6th Floor, Satpura Bhawan, Bhopal

Syllabus & Guidelines

Schedule Serial No. 25

Subject: - Diploma in clinical Biochemistry

No. of Admission: 0-50 Admission per year.







Teaching faculty with minimum qualification & experience

Non-teaching state with qualification & experience


Laboratory with Equipment








2 year (24 months)

Appendix 'A' Enclosed

As per appendix 'A'



(two year course)



(Diploma in clinical Biochemistry)

I. Faculty.

II. Eligibility for Admission.

III. Availability of seats and fee structure.

IV. Selection of Candidates.

V. Registration.

VI. Session.

VII. Training.

VIII. Examination and Assessment.

IX. Infrastructure, Accommodation, Apparatus, Furniture, Hostel, Hospital & other items.

X. Detail Syllabus.

Draft audition for the Diploma in Clinical Biochemistry (D.C.B)

I. Faculty: -

(i) Teaching:

1. Head of the Department-01.

The head of the department for diploma course shall be of the status of associate professor having at least 5 years teaching experience with M.D. Biochemistry or M.Sc Medical Biochemistry Or Ph.D medical Biochemistry

2. Assistant professor-05 A) Two- M.Sc Medical Biochemistry with one year Teaching experience or M.D. Biochemistry or Ph.D Medical Biochemistry B) Three- One each in anatomy/physiology/pathology having M.D/M.S in the subject of M.Sc in the subject with one year teaching experience

3. Demostrator/Instructor- 02 MBBS/M.Sc. BioChemistry/Medical Biochemistry

(ii) Technical and Other Staff:

1. Technicians – 03 D.M.L.T./DCB/B.Sc with 2 year experience in Medical Laboratory Technology.

2. Lab Assistant – 02 Higher secondary/10 + 2 with science biology.

3. Lab. Attendant – 02. 4. Sweeper – 02.

II. Eligibility:

10 + 2 with Science Biology with not less than 50% marks (40% in case of reserve category) conducted by the recognized board council/university.

III. Availability of seats and fee structure:

Admission may be upto 50 Candidates each year as per reservation policy of state government for SC/ST/OBC and women candidates. Fee structure as decided by the council/Institution.

IV. Selection of Candidates:

Selection of Diploma in Clinical Biochemistry shall be done strictly on merit judged on the basic of marks obtained at 10 + 2 level or in the competitive examination as decided by the council. If more than One Diploma courses are run by the Institute than admission by counselling. Age limit – minimum 17 years upto 31st Dec. of the year of admission.

V. Registration:

A candidate for Diploma courses shall registered himself/herself with the University on before the dates fixed by the university for diploma courses. Registration fee shall be payable by the candidates.

VI. Academic session:

Academic session shall start every year in month of July – August.

VII. Training:

1. The period of training for diploma in clinical Biochemistry shall be 2 years from the date of his/her admission.

2. The candidate will under take the training as a full time student in

Biochemistry department.

3 Candidate will also require participating in hospital work/emergency duties.

4. For the final examination candidates who have attended 75% of the theory & practical classes in aggregate will be allowed to only appear in the examination.

5. Minimum passing marks will be 50% in aggregates in theory and practical but not less than 40% in theory & practical separately. The Board of studies/academic council of University/Paramedical council will divide

6. Syllabus and scheme of examination from time to time.

Pattern of Examination

Ist year

Theory two paper

Practical 2 (Two)


Theory 100

Practical 100

Paper + Internal Assessment + Viva

Viva + Experiment + Record

. Anatomy and Physiology

50 + 25 + 25

50 + 25 + 25

2. Principles of Biochemistry

50 + 25 + 25

50 + 25 + 25

IInd year

Theory two papers

Practical 2 (Two)


Theory 100

Practical 100

Paper + Internal Assessment + Viva

Viva + Experiment + Record

1. Body Fluids and Hematology

50 + 25 + 25

50 + 25 + 25

2. Clinical Biochemistry

50 + 25 + 25

50 + 25 + 25

Minimum passing marks will be 50% in aggregate in the theory & practical but not less than 40% marks in theory & practical separately in each University examination.

Internal assessment in each paper will be covering the performance of the student in the sessional examination. The HOD of the dept. concerned will award these marks.

The panel of the examiner will consist of two each year. Out of which one will be internal examiner. Theory paper one will by set by external examiner and theory paper two will be set by internal examiner. Internal examiner will be head of the department concerned. Practical examination will be of six hours. Duration spread over on two days as practical Practical II & I.

IX. Infrastructure, Accommodation, apparatus, furniture, other items

(A) Accommodation: (i) Rooms/ chambers 08

Head of the department 01

Assistant Professors 02

Demonstrators 01

Technical Staff 01

Store room 01

Preparation room 01

Clerks etc. 01 08

ii) Laboratory/Museum- 03

1. Hematology/Physiology/Path. Laboratory

Facility for 50 students- microscopes, C.B.C instruments (Automatic), Hemocytometer, Haemoglobinometer, B.P instruments, sati scope, E.S.R tubes, slides, cover, slips, other glass ware etc. Required furniture tables etc.

1. Anatomy museum- with models, organ specimens, human skeleton set, chart diagrams etc.

1. Clinical Biochemistry laboratory first aid box, slides, cover slips, single pen balance, PH meter, Photoelectric calorimeter, semi auto analyzer/auto analyzer, centrifuge machines, water bath, constant temperature water bath, vortex, shaker, Owen, incubator, semi auto analyzer,

Centrifuge machine, Microscopes, distilled water plant, Triple distilled water plant, Paper and agar gel electrophoresis apparatus, flame photometer, Chromatography unit (complete) freeze

facility for demonstration of fully automated/ semi automated blood analyzer, blood gas analyzer, Elisa reader, arrangement for disposal of laboratory waste products. Experimental tables with shelf's, Almirahs, Proper ventilation (exhaust fan) glass ware and chemicals as per requirements

III) Classrooms- 02 Two classroom having capacity as per No. Of students with modern equipment OHP, slide projector, Epidiascope. Rooms, Laboratories, Museum, classroom must be well furnished with proper water supply arrangements

IV Hostel accommodation- For 40 students, 20 each for boys and girls.

V. Hospital- Institute must have minimum have 60 bedded hospital or arrangement with other hospital for clinical material and training.

Paper- I


M.M. 50 Marks

a) Anatomy: -

1. Introduction of Human body its different parts, Common anatomical terms

2. Cells, tissues, organs and body regions

3. Structural and support systems. The skin, Bones, muscles and joints

4. The nervous system, gross structure of brain, spinal cord, general sensory system, skeletal system, special sensory system.

5. Mouth, Pharynx, Thoracic cavity, the respiratory system, abdomen, structure of elementary canal.

6. Gross structure of heart and pericardium.

7. Urinary and genital system.


1. Blood composition, classification and function

2. Cardiovascular system, heart, blood vessels.

3. Respiratory system- Mechanism of respiration.

4. Excretory system- Functions of glomerulus's and tubules, composition of urine, skin functions.

5. Digestive system

6. Endocrine glands- Hormones secreted by them and their function.


Anatomy & Physiology M.M. 50 Marks

Anatomy: -

(1) Demonstration of parts of body and land marks on the surface.

(2) The skeletal system, Head & Neck.


- Demonstration of various organs within torex & abdomen.

– Respiratory systems, pleurac, heart, liver, gall bladder, peritoneum stomach & intestine.

– Spleen, pancreas & parts of urinary system


- Spinal level and site of lumber puncture.

– Surface anatomy of important organs & blood vessels.

– Identification of models like those of Brain, Heart, embryology, Kidney.


- Fixing, labeling & storage of specimens.

– Drawing diagrams & labeling.

– Demonstration of models, specimens & skeletion.


-The microscope, its usage, cleaning & maintenance.

- Identification of blood cells under Microscope. RBC, various types of WBC, pallets, Reticulocytes.

– Preparation of anti coagulants.

– Collection of blood samples to obtain plasma & serum samples.

– Ruling area of Neubaur chamber.

– Usage of RBC & WBC pipettes & wintergreen Pipette & Win robe tube.

– Estimation of Hb, preparation of blood smears, staining.

– Demonstration of blood pressure recording and pulse.

– Determination of bleeding, clotting & prothrombin Time

Paper- II

M.M. 50 Marks


1. Introduction and scope of biochemistry

2. Elementary knowledge of analytical biochemistry. Principles, functions and uses or balances, centrifuge machines, calorimeter, photoelectric calorimeters, flame photometer, spectrophotometers, Electrophoresis, Chromatography, auto analyzer, blood gas analyzer, Elisa readers.

3. Elementary knowledge of Physico-chemical concepts of some simple phenomenon. Statistics and computer application. Osmosis, Osmotic pressure, dialysis, absorption and viscosity, Donnan equilibrium, surface tension, pH. Buffers, acid base balance.

4. Chemistry of carbohydrates, fats, proteins and Nucleic acid

5. Elementary knowledge of enzymes, biological, oxidation, co-enzymes.

6. Outline of digestion, absorption and metabolism of carbohydrate, proteins and fats.

7. Importance of some minerals- sodium, potassium, calcium, phosphorous, iron, copper, chloride.

8. Nutritional aspect of carbohydrates, fats, proteins, balanced diet.

9. Chemistry of urinary/ Gall bladder stones.

PRACTICAL M.M. 50 (a) Demonstration:

Maintenance of laboratory, quality control, and first aid

Cleaning of glassware

Preparation of various solution

Single pan balance, Operation and maintenance

pH- meter components

Titration- Acid and normal solution.

Handling of calorimeters.

Distillation of water.

Paper & Agar gel electrophoresis

(B) Qualitative analysis of carbohydrate, fats and proteins.

(c) Analysis of milk and wheat flour.

(d) Serum electrolytes Na.K.Cl.Ca and P.

(e) Demonstration of automated blood analyzers. Blood gas analyzer, Elisa reader.

(f) Demonstration of disposal of laboratory waste product and infected material.

(g) Preparation of conventiantional reagents etc.

D.C.B. Second year

Theory Paper I


HEMAATOLOGY: - Introduction to Hematology, composition of blood, collection of blood sample.

– Haemopoises, anticoagulants

Red blood cells

– Leucocytes, Normal Value, WBC count, Differential count.

– Hemoglobin- Normal and Abnormal Hemoglobin. PCV. Absolute values, Anaemia. E.S.R.


- Urine Composition & Formation. Normal & Abnormal constitutions.

Sputum- Collection & AFB stain.

Semen– Method of collection, examination of semen.

GASTRIC ANALYSIS:- Composition of gastric juice, method of collection, Gastric Analysis.

STOOL: - Examination of stool, Fat in stool ova & Cyst. (Page577)



(1) Practice of drawing Blood from finger & from vein.

(2 Preparation of anticoagulants, RBC fluids. WBC fluid, fluid for ecosinophyll count & plat let counts.

(3) Emonstration of PCV, ESR.

(4 RBC count. WBC counts. Total & differential. Hb- estimation.

(5) Urine examination of stool, sputum & Semen.

Paper II



(1) Kidney function test – General consideration evaluation of KFT.

(2) Liver function test – Liver Function. Investigation of Liver Function uses of Liver Function Test.

(3) Pancreatic Function Test –Function of pan crease, pancreatic juice, and function tests.

(4) Gastric Function – General consideration, Chemical analysis of gastric contents. Clinical Significance.

(5) Test for Thyroid- Thyroid function, importance of thyroid function tests.

(6) Cardiac profile Test – General consideration cardiac function, isocheim Heart diseases, Cardiac profile test.

(B) ENZYMES: - Clinical enzymology, importance of enzymes- alkaline and Acid phosphates. Transaminases. Cretin phosphokinase Amylase.

(C) Hormones: - Hormones of Thyroid, pituitary, Adrenal Gonads, and their clinical importance.

(D) Vitamins: - Deficiency systems & clinical importance.

(E) Jaundice: - Bile pigments derangement. Different types of jaundice, General consideration & interpretation.



(1) Demonstration of Kidney function test. Gastric function test & liver function test.

(2) Demonstration of Enzyme Analysis – Acid and Alkaline phosphates, SGOT/SGPT. Lactic dehydrogenase, CPK.

(3) Lipid profile.

(4) Estimation of Blood/ serum- Glucose, G.T.T. Urea, creatinine, uric Acid, Cholesterol. Bill Rubin. Total protin & A/G Ratio, Glycosylated Hb.

(5) demostration of semi automated, Fully automated Biochemical Analyzers.(6) Demonstration/ Exposure to Radioimmuno assay laboratory.

(7) Visit to Laboratory of National Importance