MADHYA PRADESH PARAMEDICAL COUNCIL
6th Floor, Satpura Bhawan, Bhopal
Syllabus & Guidelines
Schedule Serial No. 4
Subject: - Laboratory Technician(Other type) Blood Transfusion
No. of Admission: - 0-50 Admission Per Year
Teaching faculty with minimum qualification & experience
Non teaching state with qualification & experience
Laboratory with Equipment
1 year (12 months) Technician
Appendix ‘A’ enclosed
1.Associate Professor Hemotology–1.
2.Assistant Professor Pathology–1.
Fully Equipped Hemotology Deptt.
Lecture Hall–2500 sqft.
Demonstration room –3 1250 sqft each.
25 room double seated 15’ x 20’ with kitchen dinning rooms, toilets
Qualification and experience of teaching staff as per M.C.I. norms.
SYLLABUS FOR CERTIFICATE COURSE IN MEDICAL LABORATORY TECHNOLOGY (C.M.L.T.):
1. The period of training shall extend over 12 months.
2. At the end of the course theory, practical and viva voice examination will be conducted and the successful candidates awarded certificate.
I. GENERAL LABORATORY TECHNIQUES One Month:
(a) Microscope- its components. Uses and care.
(b) Preparation and handling of glass-ware such as blowing and bending, marking capillary pipette, cleaning and sterilisation etc.
(c) Balance – Its components, uses and care.
(d) Solution and reagents. Making of
(e) Distillation of water and alcohol.
(f) Maintenance of machine- their uses and care. Refrigerator, Incubators, Centrifuge, Hot air ovens, Autoclave etc.
(g) Stock keeping and indexing of materials.
(h) Record keeping of laboratory work.
II. CLINICAL PATHOLOGY - FOUR MONTHS:
1.Collection, preservation and transportation of materials.
2. Preparation of stains and solutions.
3. Various routine staining methods- Leishman’s Gram’s, Methylene blue, Albert’s ziehl Nelson and Giemsa’s Examination of urine – physical chemical and Microscopic.
4. Examination of Faeces- Physical occult blood, microscopic.
5. Examination of sputum general appearance, Microscopic.
6. Blood examination – collection of specimen, making of thick and wet films, haemoglobin estimation; R.B.C. and W.B.C. count staining of blood slides with leishman’s stain and special stains normal and abnormal cells in blood; recticulocyte count; differential W.B.C. count; platelet count, prothrombin time; sternal marrow smear; P.C.V.; M.P.; Microfilaria; blood grouping and matching; E.S.R.; bleeding and coagulation time; red cell fragility tests.
7. Seminal fluid examination-count and smear study.
III. MICROBIOLOGY AND SEROLOGY TWO MONTHS:
1.General glass ware and care, cleaning, sterilisation, pipette making, anaerobic jars, pumps, centrifuges, staining of smears, types of loops, handling of bacteria, etc. and use of filter.
2. Media preparation – nutrient broth, nutrient agar, blood agar, D.C.A., L.L.BA., L.J. Dorsels eggs, loeffler’s etc.
3. Cultural methods – plate, fluids, slopes, spreading, anaerobic culture.
4.Methods for study of motility, biochemical reactions and slide and tube agglutination.
Bacteriological examinations of pus, C.S.F., Fluids, Swabs, tissues, maintenance of stock culture.
Agglutination tests, widal tests, Weil Felix, Paul Bunnuel, cold agglutination, preparation of antigen, suspensions and techniques.
V.D.R.L., antigen preparation and its interpretation or latex test.
IV. CLINICAL BIOCHEMISTRY – Two Months:
(a) Care of glassware, titration making of standard solutions uses of graduated glass ware, calorimetry – turbidimetry, P.H. estimation.
(b) Chemical Examination. (i) C.S.F. (ii) Blood Sugar, Urea, N.P.N. Protein. (iii) Gastric Analysis, (iv) Function tests of kidney and liver, blood urea, urine urea, van den bergh, lcteric index and turbidity tests etc.
V. BLOOD BANK – One Month:
(a) Equipments, preparation of pyrogen free distilled water and saline.
(b) Use of various forms in blood bank.
(c) Grouping of blood.
(d) Matching of blood.
(e) Collection of blood from donors.
(f) Preservation solutions.
(g) Transfusion of blood, indexing and record keeping.
VI. HISTOPATHOLOGY AND MUSSEUM – Two Months:
(a) Micro tomes – freezing, rotary and rocking.
(b) Preparation of stains.
(c) Tissue fixation, tissue processing, tissue embedding.
(d) Section cutting staining, Haematoxylin and eosin stain special stains for fat, recticulin, nerve and glycogen etc.
MUSEUM TECHNIQUES - Framing of tissues, making frames, selection of jars, making general and special preservation and solution, mounting of tissues, maintenance of tissues appearances, colour schemes slides and specimen indexing, etc.
In addition the following special lectures shall be by members of the staff of the department.
SECTION A – SPECIAL LECTURES: By senior staff:
1.General knowledge of Anatomy of Human body I (Elementary)
2. General knowledge of Anatomy of Human body II.
3. General knowledge of Elementary Human Physiology I.
4. General knowledge of Elementary Human Physiology II.
5. Knowledge of dispatch of Biological materials to out-stations.
6. Knowledge of dispatch of post, mortem material.to out stations and to Chemical Examiners.
7. General knowledge of serology and Immune bodies and their reaction.
8. Agglutination reaction and their applied laboratory importance to a technician. 9. Precipitation and flocculation reaction and their applied laboratory importance to a technician.
10. Complement fixation reaction and their applied importance in laboratory techniques.
11. Duties of a laboratories assistant in general.
12. Position of a laboratory technician in relation to the institute where he is working.
13. Relation and duties and position of a technician to the institute.
SECTION B – CLINICAL PATHOLOGY:
1. Development of R.B.C, W.B.C. and platelets.
2. Examination of bone marrow smears.
3. Staining of blood films with special stains and platelet counting.
4. Coagulation, bleeding time and R.B.C. fragility test.
5. Laboratory examination of (a) C.S.F. and (b) Semen.
SECTION C – MICROBIOLOGY AND SEROLOGY:
1. Determination of sensitivity of bacteria to antibiotics.
2. Testing of disinfectants.
3. (a) complement fixation test.
(b) Method of performing W.R., V.D.R.L., slide test tube test.
4. (a) Bacteriological examination of water.
(b) Bacteriological examination of Milk.
5. Dark ground illumination, Florescence microscopy, phase contrast microscopy.
SECTION D – BLOOD BANK:
1. History of Blood transfusion.
2. Method A.B.O. Grouping.
3. Titration of anti A & B sera, cold agglutinin, Coomb’s test.
4. Blood transfusion.
5. Maintenance of registers and different forms used in blood bank.
SECTION E – CHEMICAL PATHOLOGY (BIOCHEMISTRY):
1. Care of glassware, calorimeter, spectrophotometer and filters.
2. Use of analytical balance, solutions, Ph. And its estimation.
3. Examination of C.S.F.
4. Blood sugar; Blood urea.
5. Liver functions test and renal function test.
SECTION F – MORBID ANATOMY INCLUDING HISTOLOGY AND (POST MORTEM AND MUSEUM):
1. General introduction and study of cell structure.
2. Fixation, fixatives.
3. Dehydration, cleaning, embedding.
4. Micro tomes sectioning.
5. Staining of paraffin sections and special stains.
(a) Introduction to ABO Blood groups and Rh types. Other types of blood systems, Collection of Blood Sample for
(1) ASO Group and Rh type
(2) Cross matching.
(3) Coomb’s test – Direct and Indirect.
Preparation of grouping sera.
Preparation of ACD solution & CPS solution.
Sterilization of different equipment for blood collection.
(b) Labelling and maintenance of blood bags of records and registers.
– Blood Bank Refrigerators.
– Refrigerating centrifuge.
– Deep Freeze.
(d) Donor selection.
(e) Tests on donor’s blood
– HIV I & II.
– HB & Ag.
– Hepatitis C.
– CMV (Cytomegalovirus).
(f) Bleeding and storing of the blood.
(g) Transportation of the blood bags.
(h) Inventory control of blood & its equality control.
(i) Preparation of different components of Blood for transfusion.